Journal: eLife
Article Title: Adolescent alcohol exposure promotes mechanical allodynia and alters synaptic function at inputs from the basolateral amygdala to the prelimbic cortex
doi: 10.7554/elife.101667
Figure Lengend Snippet: Figure 3. Experimental approach. (A) Experimental timeline displaying the age of animals at each phase in the study. (B) Diagram showing the viral and retrobead labeling approach used to identify and manipulate specific neuronal populations in the prelimbic (PrL) cortex. (C) Representative images showing injection sites in the PrL (left panel, AAV2-hSyn-DIO-mCherry), basolateral amygdala (BLA, center panel, AAV5-hSyn-hChR2(H134R)-EYFP), and ventrolateral periaqueductal gray (vlPAG, right panel, green retrobeads). (D) Representative images from the PrL cortex (from left to right) of BLA terminals, mCherry-tagged parvalbumin interneurons (PVINs), and green retrobead labeled PrLPAG neurons.
Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Rattus norvegicus) Parvalbumin- Cre on a Long- Evans background Rat Resource and Research Center RRID:RRRC_00773 Male and female Recombinant DNA reagent AAV5- hSyn- hChR2(H134R)- EYFP Addgene RRID:Addgene_26973 Cat#:26973- AAV5 Recombinant DNA reagent AAV2- hSyn- DIO- mCherry Addgene RRID:Addgene_50459 Cat#:50459- AAV2 Chemical compound, drug λ-Carrageenan (low viscosity) Tokyo Chemical Industry Cat#:C2871 CAS#:9064- 57- 7 Chemical compound, drug Picrotoxin Ascent Scientific Cat#:ASC- 315 CAS#:124- 87- 8 Chemical compound, drug Kynurenic acid sodium salt Hello Bio Cat#:HB0363 CAS#:2439- 02- 3 Chemical compound, drug Tetrodotoxin (citrate) Cayman Chemical Cat#:14964 CAS#:18660- 81- 6 Chemical compound, drug 4- Aminopyridine Sigma- Aldrich Cat#:A78403 CAS#:504- 24- 5 Chemical compound, drug DL- APV Cayman Chemical Cat#:14540 CAS#:76326- 31- 3 Software, algorithm Axograph X Axograph RRID:SCR_014284 Software, algorithm Stata 15.1 StataCorp RRID:SCR_012763 Other Green Retrobeads IX Lumafluor
Techniques: Labeling, Injection
